sgrna library system Search Results


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Kuang Lung Shing library of single guide rnas (sgrnas)
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Shanghai Genechem Ltd human geckov2.0 sgrna lentiviral knockout library
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Image Search Results


Troubleshooting table

Journal: Nature protocols

Article Title: Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

doi: 10.1038/s41596-021-00669-0

Figure Lengend Snippet: Troubleshooting table

Article Snippet: To identify an sgRNA target sequence using the GenScript sgRNA database ( https://www.genscript.com/gRNA-database.html ) or other webtools, input the gene name, symbol or ID and search for human SpCas9 gRNA sequences.

Techniques: Negative Control, Ligation, Transformation Assay, Plasmid Preparation, Selection, Passaging, Clone Assay, Concentration Assay, Sequencing